Table 1. Primers used for the molecular detection E. coli and Vibrio spp.

From: Molecular detection and antibiogram profiles of Escherichia coli and Vibrio cholerae isolated from raw vegetables in the northern district of Bangladesh

Name of primers

Sequence (5′-3′)

Amplicon

size (bp)

reference

16S rRNA

 

F: AATTGAAGAGTTTGATCATG

704 bp

Sarker et al. (2018)

R: CTCTACGCATTTCACCGCTAC

groEL 

F: TCCARAACATGGGCGCACAA

1117 bp

Hossain et al.

(2014)

R: ACGTTTTGYTCTTCGTTGTCRC

 

Reference:

Sarker MAR, Haque MM, Rifa RA, Ema FA, Islam MA and Khatun MM, 2018. Isolation and identification of bacteria from fresh guava (Psidium guajava) sold at local markets in Mymensingh and their antibiogram profile. Veterinary World, 11(8): 1145. https://doi.org/10.14202/vetworld.2018.1145-1149

Hossain MT, Kim YR and Kong IS, 2014. PCR–restriction fragment length polymorphism analysis using groEL gene to differentiate pathogenic Vibrio species. Diagnostic Microbiology and Infectious Disease, 78: 9-11. https://doi.org/10.1016/j.diagmicrobio.2013.10.005

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